计算机科学与探索 ›› 2010, Vol. 4 ›› Issue (2): 97-107.DOI: 10.3778/j.issn.1673-9418.2010.02.001

• 综述·探索 • 上一篇    下一篇

串联质谱的蛋白质序列鉴定技术综述

乔彦涛1,3+,缪佳铮2,孙世伟3,刘金刚1,卜东波3   

  1. 1. 首都师范大学 计算机科学联合研究院,北京 100048
    2. 浙江工商大学 食品与生物工程学院,杭州 310018
    3. 中国科学院 计算技术研究所 计算生物研究组,北京 100190
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-02-15 发布日期:2010-02-15
  • 通讯作者: 乔彦涛

Review on Protein Identification Approaches Based on Tandem Mass Spectrometry

QIAO Yantao1,3+, MIAO Jiazheng2, SUN Shiwei3, LIU Jingang1, BU Dongbo3   

  1. 1. Join Faculty of Computer Scientific Research, Capital Normal University, Beijing 100048, China
    2. School of Food Science and Technology, Zhejiang Gongshang University, Hangzhou 310018, China
    3. Bioinformatics Group, Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100190, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-02-15 Published:2010-02-15
  • Contact: QIAO Yantao

摘要: 蛋白质组学的主要目的是鉴定出生物体内的蛋白质的种类和数量。为达到这个目的,人们开发了多种蛋白质鉴定算法,包括数据库搜索方法、De Novo方法、PST(肽段序列标签)方法和质谱数据库方法。首先介绍了质谱仪中肽段断裂机理的研究,以及相关的质谱鉴定方法,然后综述了当前常用的蛋白质鉴定方法,分析了这些方法的优缺点,最后提出了自己的见解和展望。

关键词: 蛋白质组学, 串联质谱, 数据库搜索方法, De Novo算法, 质谱数据库方法

Abstract: One of the main objectives of proteomics is to identify and quantify proteins in a tissue or a cell. Tandem mass spectrum has been proved to be a powerful tool to achieve this goal. This paper presents a review on the protein identification approaches based on tandem mass spectrometry, including database searching methods, De Novo methods, PST (peptide sequence tag) methods, and spectra library methods. It also introduces the research on peptide fragmentation principle, amino acids neutral losses, and peptide identification. Finally, the viewpoints on the development of mass spectrum techniques are described.

Key words: proteomics, tandem mass spectrum, database searching method, De Novo method, spectra library method

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